Table 1.

Changes in expression of cholesterol biosynthesis and uptake genes and steroidogenic genes in cells acclimated to 1 nM testosterone exposure. The data are expressed as fold of change relative to the values in control cells. The numbers in bracket represent the range converted from the ΔΔCt values (mean ± SD).

Gene\Model		VCaP	LNCaP	LAPC4
Cholesterol synthesis	ACAT2	1.0	3.3 (3.1–3.4)**	2.1 (1.9–2.3)*
	HMGCS1	− 1.1	2.1 (2.0–2.1)**	2.8 (2.7–2.9)**
	HMGCR	1.6	1.9	2.1 (2.0–2.1)**
	MVK	1.5	2.3 (1.9–2.6)*	2.4 (2.3–2.5)**
	PMVK	1.6	2.1 (2.0–2.2)**	1.8
	MVD	1.5	2.0 (1.9–2.0)**	2.6 (2.5–2.6)**
	FDFT1	1.3	3.2 (3.1–3.3)**	2.4 (2.3–2.5)**
	SQLE	1.9	1.7	2.1 (2.0–2.1)**
uptake Cholesterol	SR-BI	1.5	4.0 (3.8–4.2)**	1.6
	LDLR	1.6	2.5 (2.5–2.5)**	1.5
	LOX1	−9.0 (8.1–10.0) **	1.5	1.9
	SREC	− 4.7 (3.8–5.8) *	1.2	1.5
	STAR	− 4.7 (3.9–5.6) **	2.3 (2.1–2.4)**	1.8
steroidogenesis	CYP11A1	− 8.2 (5.6–11.8) *	1.3	1.7
	HSD3B2	3.9 (3.6–4.3) **	1.2	1.9
	CYP17A1	− 11.3(8.9–14.4)**	− 2.5 (2.4–2.6)**	− 1.9
	AKR1C3	− 1.3	− 1.3	6.9 (5.0–9.6)*
	HSD17B3	− 3.1 (1.6–5.7) *	− 5.6 (2.8–11.0)*	1.8
	SRD5A1	1.1	1.9	3.0 (2.8–3.3)**
	SRD5A2	− 12.4(9.5–16.3)**	− 7.5 (5.2–10.8)*	1.0
	SRD5A3	− 1.8	2.2 (2.0–2.3)**	1.6
	AKR1C2	− 2.5 (2.3–2.8)**	− 1.8	5.6 (3.5–8.8)*
	RDH5	1.0	2.3 (2.1–2.5)**	1.3

^*p<0.05;

^**p<0.005, paired student’s t-test.