Table-2.
Primers used in this study.
| Gene | Forward (F) or Reverse (R) | Primer Sequence (5' to 3') | Product size (bp) | Annealing temp. (°C) | Extention time (sec) |
|---|---|---|---|---|---|
| Adh | F | TGCACCACCAACTGCTTAG | 152 | 58 | 30 |
| R | GATGCAGGGATGATGTTC | ||||
| Ifi44 | F | GAGAGAACAGGGAATGAAGAAGGC | 134 | 52.4 | 30 |
| R | CCAACAGAATTGCGATTGGTCC | ||||
| Ifi27 | F | CCATAGCAGCCAAGATGATGTCTG | 121 | 55 | 30 |
| R | GCATTTGTTGATGTGGAGAGTCC | ||||
| Oas2 | F | AAAACCAACCGCTCCCAGTTCGTC | 488 | 57.1 | 30 |
| R | GCAATGTCAAAGTCATCTGTGCC | ||||
| GTPase2 | F | CTTCCACCTGCTTGTTCTTTGG | 266 | 55.4 | 30 |
| R | TCACAGTTTCCTCAGTGCTGGG | ||||
| Rsad2 | F | CAATCACACCCAGCAGCAGTTAG | 209 | 54.4 | 30 |
| R | AGCGATGCCTCAGAACACAGTG | ||||
| Mx1 | F | CAGCACCTGAAAGCCTACTACCAG | 135 | 53.6 | 30 |
| R | GGTGTCCTGTAAAAGCTGAAGCATC | ||||
| Oasl2 | F | TTACAGAACAGCCAGAGCTATACGG | 548 | 56.1 | 40 |
| R | CAAGGGAGATAGATTTACGTCCACG | ||||
| IRF7 | F | ACACCATCTACCTGGGTTTTG | 243 | 54 | 60 |
| R | TTGGGATTCTGAGTCAAGGC | ||||
| IFN-α2 | F | CATCTGCTGCTTGGAATACAACC | 197 | 56.7 | 30 |
| R | GGGGCTGTGTTTCTTCTCTCTCAG | ||||
| IFN-α4 | F | TCAATGACCTCAAAGCCTGTGTG | 211 | 56.6 | 30 |
| R | CACTCCTCCTCACTCAGTCTT | ||||
| IFN-β | F | AAGAGTTACACTGCCTTTGCCATC | 138 | 53.2 | 30 |
| R | AAACACTGTCTGCTGGTGGAGTTC |