Agarose (1.5%) gel electrophoresis of nested PCR products. M, 50 bp ladder (Fermentas); lane 1, negative control (distilled water); lane 2, L. turanica; lane 3, L. gerbilli; lane 4, L. major; lanes 2–4 are reference strains; lane 5, L. turanica; lane 6, L. gerbilli; lane 7, L. major; lane 8, mixed infection of L. major and L. turanica; lane 9, mixed infection of L. major and L. gerbilli; lane 10, mixed infection of L. major, L. gerbilli and L. turanica. Lanes 5–10 are from skin samples of Rhombomys opimus rodents (field samples).