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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Exp Parasitol. 2010 Jun 8;126(4):552–556. doi: 10.1016/j.exppara.2010.06.003

Table 2.

Detection of Leishmania parasites from rodent skin samples by direct examination, first-round PCR and nested PCR.

Diagnosis No. of positive samples/no. of examined samples % Positive
Clinical examination
Direct examination 21/95 22.1
First-round PCR for samples positive by direct examination 12/21 57.1
First-round PCR for samples negative by direct examination 7/74 9.5
First-round PCR for all samples 19/95 20
Nested PCR for samples positive by direct examination 21/21 100
Nested PCR for samples negative by direct examination 27/74 36.5
Nested PCR for all samples 48/95 50.5