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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: RNA Biol. 2009 Jul 20;6(3):293–304. doi: 10.4161/rna.6.3.8497

Figure 4.

Figure 4

In vivo 32P radiolabeling of young, G0 arrest and senescent HS74 fibroblasts. Cells were incubated overnight in 0.5 mCi/ml 32P-ortho-phosphate. Total protein was isolated and endogenous hnRNP A1 was immunoprecipitated using 4B10 antibody. hnRNP A1 phosphorylation levels were determined by autoradiography following 12% SDS-PAGE. 300 μg of total lysates isolated from IMR-90 cells were immunoprecipitated using the 4B10-hnRNP A1 antibody, subjected to 12% SDS-PAGE and probed with the 4B10-hnRNP A1 antibody. For input, total lysates were subjected directly to western analysis. Membranes were stripped and reprobed with β-actin as a loading control.