Fig. 12.

Effects of DksA and ppGpp on in vitro transcription from the pS1, pS2 (a–d) and λp _L (e–g) promoters. Representative results are shown on autoradiograms (a, b, e and f). The presence and concentrations of particular factors in the reaction mixtures are indicated and positions of bands corresponding to certain transcripts are shown. Summaries of the results (mean values from three experiments with error bars indicating SD) are shown at the diagrams (c, d and g). The presence of following factors in the reaction mixtures is shown: DksA (diamonds), ppGpp (squares), DksA and ppGpp (triangles)