Fig. 6.

Interaction of Eσ⁷⁰ with the promotor region of the pcnB gene as assessed by DNase I footprinting. A ³²P-labeled 0.3 kb DNA fragment was incubated with 0 nM (negative control, lanes 1 and 8), 10 nM (lane 2), 50 nM (lane 3), 100 nM (lane 4), 250 nM (lane 5), 500 nM (lane 6) or 1,000 nM (lane 7) Eσ⁷⁰. The footprinting experiments were performed as described in “Materials and methods”. The −10 and −35 sequences of p1, pB and p2 are indicated