Figure 1.

CIP29 associates with the human TREX complex. (A) Western of nuclear extract (1 μL) using an antibody against CIP29. Markers in kilodaltons are indicted. (B) IPs from nuclear extract using the CIP29 antibody (lane 2) or a negative control antibody (fSAP130) (lane 3) followed by Western with the CIP29 antibody; 1.5% of nuclear extract input is shown in lane 1. (C) A mixture of in vitro translated (IVT) CIP29 and luciferase (lane 1) was used for IP with the CIP29 antibody (lane 2) or a negative control antibody (fSAP130) (lane 3). (D, left panel) Coomassie-stained gel of IPs using indicated antibodies. H and L indicate the heavy and light chains of antibodies, respectively. Molecular weight markers in kilodaltons are shown. IPs in the left panel were used for Westerns with the indicated antibodies (shown in the right panel).