Figure 2.

CIP29 is present in nuclear speckle domains, and its efficient recruitment to mRNA is both splicing- and cap-dependent (A) IF of HeLa cells was performed using CIP29, SC35, or Aly antibodies. (B) Same as A, except using Cos cells and CIP29 and Aly antibodies. (C) IPs of spliced or Δi AdML mRNA with indicated antibodies. The inputs (25%) (shown in lanes 1,9) and IPs were run on a 6.5% denaturing gel. (D) Same as C, except that capped and uncapped AdML spliced mRNA was used. Quantitations of three independent experiments are shown. Error bars represent standard deviations (n = 3).