Figure 6.

ATP-dependent remodeling of the TREX complex in nuclear extract. (A) Nuclear extracts were separated on a gel filtration column in the presence of ATP followed by Westerns of fractions using the indicated antibodies. (B) IPs from +ATP fractions were used for Western analysis with the indicated antibodies. (C) Same as A, except nuclear extracts were separated in the absence of ATP. (D) IPs from −ATP fractions were used for Western analysis with the indicated antibodies. (E) IPs from −ATP fractions with ATP added back were used for Western analysis with the indicated antibodies. (*) Antibody heavy chain. The 60S and 30S size markers are from the spliceosome and hnRNP complex, respectively.