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. 2010 Aug 31;2010:630267. doi: 10.1155/2010/630267

Figure 1.

Figure 1

SDS-PAGE (12%) profile showing the expressed and purified recombinant Cry1Ia12 protein. Lane 1: Marker BenchMark Protein Ladder (INVITROGEN). Lane 2: E. coli crude extract induced with 1 mM IPTG after 16 h expressing recombinant Cry1Ia12 protein, sonicated and centrifuged. Arrows indicate the expressed 74-kDa recombinant Cry1Ia12. Lane 3: Noninduced E. coli strain BL21Star (DE) containing the expression vector without insert was used as a negative control of the expression. Lane 4: Recombinant Cry1Ia12 protein purified by using Ni-NTA column. Arrow shows the expected purified 74-kDa recombinant Cry1Ia12 protein.