Fig. 1.

Resveratrol protected DA neurons against LPS-induced neurotoxicity. Rat primary mesencephalic neuron-glia cultures were seeded in 24-well culture plates at 5 × 10⁵/well and then pretreated with various concentrations of resveratrol (15–60 μM) for 30 min before the addition of 10 ng/ml LPS. Seven days later, the LPS-induced dopaminergic neurotoxicity was determined by [³H]DA uptake assay (A) and the quantification of TH-positive neurons after immunostaining of DA neurons with an anti-TH antibody (B). Representative images of immunostaining from three independent experiments are shown (C). Scale bar, 100 μm. Results are the mean ± S.E.M. from three independent experiments performed in triplicate, and data from [³H]DA uptake assay are expressed as a percentage of the control cultures. #, p < 0.05 compared with control cultures; *, p < 0.05 compared with LPS-treated cultures.