Fig. 8.

Resveratrol prevented LPS-induced activation of microglia NF-κB signaling pathway. Primary microglia-enriched cultures were pretreated with resveratrol (60 μM) for 30 min and then incubated with LPS (10 ng/ml) for 15 min. The whole-cell lysates were analyzed by Western blotting assay (A). The ratio of densitometry values of phosphorylated p65 and IKKβ compared with total p65 and IKKβ and phosphorylated IκBα and total IκBα relative to β-actin was analyzed and normalized to each respective control group (B). Results are the mean ± S.E.M. from three independent experiments. #, p < 0.05 compared with control cultures; *, p < 0.05 compared with LPS-treated cultures.