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LC/MS analysis of CCG-4986-treated RGS8. Purified human RGS8 was incubated with 100 μM CCG-4986 for 1 h at room temperature. RGS8 (50 pmol) was subject to reversed-phase high-performance LC using a C18 column. The deconvoluted chromatogram shows unmodified protein, a singly adducted RGS8 (+153 mol. wt.), and the doubly modified RGS8 (+306 mol. wt.), indicating that both of the cysteines present in RGS8 can be modified by CCG-4986.
