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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Nature. 2010 Jan 7;463(7277):98–102. doi: 10.1038/nature08652

Figure 2.

Figure 2

Functional properties of the light-driven proton pump Arch in neurons. (A) Photocurrent of Arch measured as a function of ionic composition (575 ± 25 nm light, 7.8 mW/mm2), showing no significant dependence of photocurrent on concentration of Cl− or K+ ions (N = 16, 8 and 7 neurons, from left to right). (B) Arch proton photocurrent vs. holding potential (N = 4 neurons). (C) Intracellular pH measurements over a one minute period of continuous illumination and simultaneous imaging (535 ± 25 nm light, 6.1 mW/mm2,) using SNARF-1 pH-sensitive ratiometric dye (N = 10 – 20 cells per datapoint). (D) Trypan blue staining of neurons lentivirally-infected with Arch vs. wild-type (WT) neurons, measured at 18 days in vitro (N = 669 Arch-expressing, 512 wild-type, neurons). (E) Membrane capacitance, (F) membrane resistance, and (G) resting potential in neurons lentivirally-infected with Arch vs. wild-type (WT) neurons, measured at 11 days in vitro (N = 7 cells each).