Figure 4.

SeHex and SeSP1 mRNA expression on the condition of starvation. The day-2 fifth instar larvae were used as experimental insects in the treatment of starvation. In the experiment of starvation, two experimental group insects were hungered for 6 h and 12 h, respectively. Followed these insect continue to rear at 25 ± 1°C with an L14:D10 photoperiod using an artificial diet. The insects were observed at 0 h (before treatment), 6 h, 12 h, 24 h, 36 h, 48 h and 72 h after treatment. Every three to five lively larvae were randomly selected and stored at -80°C for subsequent RNA extraction. (A) for SeHex in starvation, (B) for SeSP1 in starvation. The housekeeping gene β-actin was used as a reference.