Table 1.

Primers for cDNA cloning, Northern blotting, semi-quantitative RT-PCR, dsRNA synthesis and detection of SeHex and SeSP1 RNAi

PCR Fragme	Primers
	Name	Directiona	Typeb	Nucleotide Sequence (5'-3')
1	Hex-DF1	F	D	TGG ARM GHC TGT CYA ACG
	Hex-DF2	F	D	ACA AKG GMA TBH HST TCC
	SP1-DF1	F	D	GGC ATG TTC DTV TAT GC
	SP1-DF2	F	D	TAC CCN TAC TWC TTC GTC
	Hex-DR1	R	D	GAA BGG CAT RCC DCC GAC
	Hex-DR2	R	D	GTC CAG CTS SAW GAA GTT
	SP1-DR1	R	D	CGT ACT TGG GNC CAN DGA AG
	SP1-DR2	R	D	TGA ANG GVT GRT GRT TGA G
2 RACE	3-CDS	R	O	AAG CAG TGG TAT CAA CGC AGA GTA C(T)30VN
	NUP	F	A	AAG CAG TGG TAT CAA CGC AGA GT
	Hex-5R1	R	G	GCT GAT GTC AAT ACG TTC ACC GAG
	Hex-5R2	R	G	CGT AAC CCT GTT CGA TGG CGT CAC
	SP1-5R1	R	G	GAT GAC ATG GCT ATC GAC GA
	SP1-5R2	R	G	CGT AGG GAG CAG GCA GAG TG
	Hex-3F1	F	G	GTG AGC GTG TTG GTA CAG CAT CC
	Hex-3F2	F	G	GAG TGA AGT TGC TAA GAC CGT TC
	SP1-3F1	F	G	CAA CTG ACA AGC TAG TGA CC
	SP1-3F2	F	G	CGC TGA GAT GAA GAA GAA GC
3 probe	Hex-FP	F	G	CGT TTG CAC GAG GTC TTC C
	Hex-RP	R	G	GGC TTC TTC TTC ATT CAT GTG C
	SP1-FP	F	G	GAC CAA GGC AGC GAA TGA CC
	SP1-RP	R	G	CAG GGC GTT GGT GAT ATC CA
4 dsRNA	dsHex-F	F	G	GAC AGC CAC GGC TAT GAG ATT CC
	dsHex-FT	F	G	GGA TCC TAA TAC GAC TCA CTA TAG GNG ACA GCC ACG GCT ATG AGA TTC C
	dsHex-R	R	G	CCT TAG AAA CAT TGG TAG GCT CG
	dsHex-RT	R	G	GGA TCC TAA TAC GAC TCA CTA TAG GNC CTT AGA AAC ATT GGT AGG CTC G
	dsSP1-F	F	G	GAC TGG AAA GAA CAC CAT CGT C
	dsSP1-FT	F	G	GGA TCC TAA TAC GAC TCA CTA TAG GNG ACT GGA AAG AAC ACC ATC GTC
	dsSP1-R	R	G	GGT CCT TCC TGT ACA CCA TCA C
	dsSP1-RT	R	G	GGA TCC TAA TAC GAC TCA CTA TAG GNG GTC CTT CCT GTA CAC CAT CAC

^a F, forward, R, reverse; ^b D, degenerate primer; G, gene-specific primer; A, nested universal primer; O, 3'-RACE CDS primer.