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Amino acid usage is correlated with optimal growth temperature across prokaryotes. Correlation between a measure of amino acid usage bias that is related to protein stability, ERK = E + R + K - D - N - Q - T - S - H - A, and optimal growth temperature in 204 prokaryotes (Pearson's R = 0.72, p = 10-15; Spearman's ρ = 0.46, p = 10-12). See Additional file 1: Supplemental Figure S1 for a correlated alternative measure of amino acid usage bias.
