Figure 2.

Intrinsic resistance of XMRV to protease inhibitors (PIs). For XMRV_VP62+LtatSN (shaded bars), HTX/LtatSN cells were infected with virus derived from the pVP62 clone, seeded into microtiter plates, and immediately treated with the indicated doses of PIs. For HIV-1_NL4-3 (solid bars), 293T/17 cells were seeded into microtiter plates, transfected with plasmid DNA encoding the full-length NL4-3 molecular clone, and treated with the indicated concentrations of each PI. The same PI stocks were used to treat both sets of virus-producing cultures. Supernatants from PI-treated HTX and 293T/17 cultures were then diluted and plated onto MAGIC-5A indicator cells to quantify infectious particles. Bars represent the percentage of β-gal⁺ FFU in supernatants from the PI-treated cultures, relative to untreated controls, and are the means ± standard deviations from two independent experiments with two or more determinations of FFU per drug treatment per experiment. See List of Abbreviations for drug names.