Table 2.
Sequence and functions of C. botulinum candidate biomarkers
| Rank | Biomarker Sequence | Algorithm(s) | Protein Annotation | |
|---|---|---|---|---|
| 1 | EAEYIFGNFGK | X ∩ M ∩ S | Unknown | |
| EDLTNVFDLSER | X ∩ M ∩ S | Unknown | ||
| ENLITAPENTTIGEAK | X ∩ M ∩ S | Metabolism Multiple pathways | ||
| GMDTLLESFIK | X ∩ M ∩ S | Metabolism Multiple pathways | ||
| INEFPEILEYK | X ∩ M ∩ S | Enzyme Families | ||
| MNEIMQDDTLER | X ∩ M ∩ S | Carbohydrate Metabolism | ||
| TIVSLDEIEIK | X ∩ M ∩ S | Translation | ||
| YANIADYLSLGGK | X ∩ M ∩ S | Unknown | ||
| 2 | AAGLEIGETGAIK | X ∩ M | Unknown | |
| AFIESVEEALEGGEK | X ∩ M | Replication and Repair | ||
| GAYVLNKEEIEK | X ∩ M | Metabolism Multiple pathways | ||
| GEPIVLDNGAVAGQAFR | X ∩ M | Replication and Repair/Cell motility | ||
| IMNDFSMLASK | X ∩ M | Unknown | ||
| NDIVVVSPDLGSVTR | X ∩ M | Metabolism Multiple pathways | ||
| QAATAIDEYLSK | X ∩ M | Metabolism Multiple pathways | ||
| TSAEGIEIVAK | X ∩ M | Amino Acid Metabolism | ||
| DLSTNSWTMIR | X ∩ S | Replication and Repair | ||
| IIDMNNAAIDEGVNAIVK | X ∩ S | Unknown | ||
| TTTIPSMVEALSR | X ∩ S | Translation | ||
| 3 | AMESILVVIPAEK | X | Unknown | |
| CLVAEEETGLTTR | X | Metabolism Multiple pathways | ||
| EALTEYLLNMSTR | X | Metabolism Multiple pathways | ||
| EGSFIYVIGPK | X | Energy Metabolism | ||
| GIENVNVFTVR | X | Unknown | ||
| GLEVQEEVLNK | X | Nucleotide Metabolism | ||
| GTNIVNIIPIENNEK | X | Replication and Repair | ||
| GTNIVNLIPIENNEK | X | Replication and Repair | ||
| IGNIVEHEETPQISGMIK | X | Translation | ||
| ISTVGDVVDYIK | X | Unknown | ||
| KWDEDKFEEVMK | X | Unknown | ||
| TFGVELEDEPSGK | X | Amino Acid Metabolism | ||
| VGIAHNVTPETVEK | X | Amino Acid Metabolism | ||
| WYIVDAADKPLGR | X | Translation | ||
| LGLAEDEAIESK | M | Membrane Transport | ||
| MIGYDIFENEEAK | M | Carbohydrate Metabolism | ||
| MIGYDLFENEEAK | M | Carbohydrate Metabolism | ||
| QVLSFVTEETVVQR | M | Unknown | ||
| SMPALITAISELNQPR | M | Unknown | ||
| TRFETNLAVANHLVDK | M | Translation | ||
X = X!Tandem, M = Mascot, S = Sequest and ∩ = intersection. The first column indicates the rank of the biomarkers. Rank 1 markers have higher confidence than the other markers due to the concordance of the search algorithms in their identification. The second column shows the amino acid sequence of the candidate biomarker. Column 3 shows the algorithm(s) used to identify the candidate biomarker. Where there is no algorithm intersection, the candidate biomarker was exclusively identified by the single algorithm. The final column indicates the functional classification of the proteins (of the listed peptides) obtained from KEGG [21]. The biomarkers fall into 4 different categories: Metabolism, Genetic Information Processing (Replication and Repair, Cell motility and Translation), Enviromental Information Processing (Membrane Transport) and Unknown.