Figure 3.

AMA1-14e is as active in ELISpot as peptide pools and minimal epitopes. PBMC from each volunteer were tested in ELISpot assays with Ap1-12 (mixture of 153 15-mer overlapping peptides), AMA1-14e (mixture of 14 minimal epitopes), Ap1 or Ap10, and individual epitopes (consistent with that volunteer's HLA supertype - NEVVVKEEY is E14 and HPKEYEYPL is E3). AMA1-14e was as active as Ap1-12 with v002 and v005 and nearly as active with v001. Bars represent standard deviation of the mean response (duplicates).