Table 2.
AMA1 peptides used in ELISpot and ICS assays
| Pool | Amino acids | Number peptides | Class II T epitope | Residues | T epitope sequence |
|---|---|---|---|---|---|
| Ap1 | 1-63 | 13 | PL186 | 14-35 | EFTYMIFNGRGQNYWEHPYQKS |
| PL187 | 41-51 | INEHRPKEY | |||
| Ap2 | 53-115 | 13 | PL188 | 92-103 | NLFSSIEIVERS |
| Ap3 | 105-167 | 13 | |||
| Ap4 | 157-219 | 13 | PL189 | 188-204 | PLMSPMTLDEMRHFYKD |
| Ap5 | 209-271 | 13 | PL190 | 218-229 | SRHAGNMIPDND |
| PL191 | 259-271 | NGPRYCNKDE | |||
| Ap6 | 261-323 | 13 | PL192 | 279-288 | AKDISFQNYT |
| Ap7 | 313-375 | 13 | PL171 | 348-366 | DQPKQYEQHLTDYEKIKEG |
| Ap8 | 365-427 | 13 | PL193 | 390-402 | YKSHGKGYNWGNY |
| Ap9 | 417-479 | 13 | PL172 | 444-461 | SLYKNEIMKEIERESKRI |
| Ap10 | 469-531 | 13 | |||
| Ap11 | 521-583 | 13 | PL194 | 527-538 | EYKDEYADIPEH |
| PL173 | 571-588 | GNAEKYDKMDEPQHYGKS | |||
| Ap12 | 573-622 | 10 | PL173 | 571-588 | GNAEKYDKMDEPQHYGKS |
PfAMA1 peptide sequences and residue numbers were based on those of the P. falciparum clone FC27 Gene Bank ID 810891. Previously identified class II AMA1 epitopes (see text) were distributed among peptide pools, except pools 3 and 10. Seven immunodominant pools are indicated in bold type.