Table 3.
ELISpot IFN-γ activity of AMA1 peptide pools and individual 15-mer peptides within these pools
| Pool | Vol. | sfc/m | 15-mer peptide | Amino acid number | Sequence | sfc/m | Peptide number |
|---|---|---|---|---|---|---|---|
| Ap1 | 001 | 119 | Ap1-3 | 9-23 | LLSAFEFTYMINFGR | 48 | 1 |
| Ap1 | 001 | 119 | Ap1-9 | 33-47 | QNSDVYRPINEHREH | 48 | 2 |
| Ap1 | 002 | 161 | Ap1-11 | 41-55 | INEHREHPKEYEYPL | 180 | 3 |
| Ap1 | 005 | 325 | Ap1-11 | INEHREHPKEYEYPL | 322 | ||
| Ap1 | 002 | 161 | Ap1-12 | 45-59 | REHPKEYEYPLHQEH | 66 | 4 |
| Ap1 | 005 | 325 | Ap1-12 | REHPKEYEYPLHQEH | 118 | ||
| Ap1 | 001 | 119 | Ap1-13 | 49-64 | KEYEYPLHQEHTYQQ | 42 | 5 |
| Ap4 | 005 | 208 | Ap4-5 | 173-187 | NQYLKDGGFAFPTE | 50 | 6 |
| Ap4 | 002 | 208 | Ap4-9 | 189-203 | LMSPMTLDEMRHFYK | 189 | 7 |
| Ap4 | 005 | 310 | Ap4-9 | LMSPMTLDEMRHFYK | 395 | ||
| Ap4 | 002 | 208 | Ap4-10 | 193-207 | MTLDEMRHFYKDNKY | 82 | 8 |
| Ap4 | 005 | 208 | Ap4-10 | MTLDEMRHFYKDNKY | 118 | ||
| Ap4 | 005 | 208 | Ap4-11 | 197-211 | EMRHFYKDNKYVKNL | 65 | 9 |
| Ap7 | 001 | 131 | Ap7-3 | 310-324 | EDIPHVNEFPAIDLF | 139 | 10 |
| Ap7 | 001 | 131 | Ap7-4 | 314-328 | HVNEFPAIDLFECNK | 96 | 11 |
| Ap7 | 001 | 131 | Ap7-7 | 336-350 | CNKLVFELSADQPK | 77 | 12 |
| Ap8 | 012 | 138 | Ap8-6 | 384-398 | FKADRYKSHGKGYNW | 143 | 13 |
| Ap8 | 001 | 78 | Ap8-11 | 405-419 | ETQKCEIFNVKPCL | 48 | 14 |
| Ap8 | 005 | 156 | Ap8-11 | ETQKCEIFNVKPCL | 218 | ||
| Ap8 | 008 | 78 | Ap8-11 | ETQKCEIFNVKPCL | 48 | ||
| Ap8 | 005 | 156 | Ap8-12 | 409-423 | CEIFNVKPTCLINNS | 127 | 15 |
| Ap10 | 001 | 172 | Ap10-13 | 517-531 | TSNNEVVVKEEYDE | 233 | 16 |
| Ap10 | 012 | 193 | Ap10-13 | TSNNEVVVKEEYDE | 250 |
A total of 16 individual peptides were identified from a total of 91 15-mer peptides from seven active pools. Peptides Ap1-11, Ap1-12, Ap4-9, Ap4-10, Ap8-11 and Ap10-13 stimulated strong responses in more than one volunteer.