Table 5.

Predicted CD8+ T cell-restricted epitopes within AMA1 15-mer peptides specific for each volunteer

Peptide number	Predicted epitope	Amino acid number	IC50 nM	HLA restriction	HLA supertype	Epitope number
1	LLSAFEFTYMINFGR	13-21	5	B*1801	B44	E1
2	QNSDVYRPINEHREH	35-44	14991	B*4402	B44	E2
3	INEHREHPKEYEYPL	47-55	18	B*0801	B08	E3
4	REHPKEYEYPLHQEH
5	KEYEYPLHQEHTYQQ	51-59	39	B*1801	B44	E4
6	NQYLKDGGFAFPTE	175-183	142	B*0801	B08	E5
7	LMSPMTLDEMRHFYK	194-202	17	A*0101	A01	E6
8	MTLDEMRHFYKDNKY
9	EMRHFYKDNKYVKNL	198-207	4742	A*0101	A01	E7
10	EDIPHVNEFPAIDLF	327-335	7	B*1801	B44	E8
11	HVNEFPAIDLFECNK
12	CNKLVFELSADQPK	339-346	5708	A*0201	A02	E9
13	FKADRYKSHGKGYNW	389-397	201	A*3002	A01	E10
14	ETQKCEIFNVKPCL	405-414	142	A*6802	A02	E11
	ETQKCEIFNVKPCL	406-414	919	A*0201	A02	E12
15	CEIFNVKPTCLINNS	410-419	617	A*6802	A02	E13
16	TSNNEVVVKEEYDE	520-528	15	B*1801	B44	E14

The 15-mer peptides that were recognized by the volunteers (Table 3) were analysed by NetMHC web-based software to predict potential high affinity HLA binding by minimal CD8+ T cell epitopes within each 15-mer. Each minimal epitope was specific for a known HLA allele within each supertype. Those minimal epitopes with the strongest binding affinities for the HLA supertype of each volunteer were selected. The Table shows the minimal epitopes in bold. Two different HLA-restricted epitopes were predicted with peptide 14.