TABLE 1.

Ceramide concentrations (nanograms per milligram of protein) in mice fed low-fat or high-fat diet with or without amitriptyline treatment measured by liquid chromatography/mass spectrometry (n = 4–6)

Ceramide	LFD	LFD + Ami	HFD	HFD + Ami
C16	0.3 ± 0.1	0.4 ± 0.4	1.3 ± 1.0	0.3 ± 0.2
C18	0.5 ± 0.2	2.6 ± 2.6	1.6 ± 0.9	0.6 ± 0.2
C20	1.2 ± 0.2	1.7 ± 0.2	6.6 ± 1.1*	4.0 ± 0.3 †
C22	9.7 ± 1.3	13.5 ± 3.5	31.5 ± 9.0*	20.2 ± 4.2
C24	35.7 ± 9.8	27.7 ± 9.8	61.5 ± 1.5*	46.6 ± 8.2
Total	47.4 ± 9.2	45.7 ± 5.8	102.5 ± 11.1*	71.8 ± 8.3 †

^* P < 0.05, significant difference compared with the values from mice receiving the LFD.

^† P < 0.05, significant difference compared with the values from mice receiving the HFD.