Calculation of the IC50 values of the
AR01 and AR03 compounds. IC50
values of the top hit compounds were
determined using the HTS assay. A range of
concentrations of each of the compounds were
tested with 0.35 nM Ape1. The percentage of
inhibition of Ape1's activity was calculated
using the rates of reaction for each of the
concentrations of the compounds compared
against the percentage of activity of Ape1
alone. The assays for each compound were
performed three individual times. SigmaPlot
software (see equation described under
Materials and
Methods) was used to calculate the
values, and they are presented here is a
semilog plot of the IC50 value
determination. A,IC50 value curve
for AR01. B, IC50 value curve for
AR03. The p values were
calculated using the Student's
t test comparing lanes with
inhibitor with lane with no inhibitor
(DMSO), *, p ≤
0.03; **, p ≤
0.0005; and ***, p
≤ 0.0001.