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. 2010 Sep;334(3):753–760. doi: 10.1124/jpet.110.165563

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Fig. 7. — Immunofluorescence of Ca_v3.1 (α1G) T-type VGCC subunit (green) in freshly isolated endothelial cells from small pulmonary arteries harvested from control (top) and CH (middle and bottom) rats (magnification: ×630). Top and middle, images for control (top) and CH (middle) show detectable Ca_v3.1 channel subunit fluorescence (indicated by yellow arrows). Bottom left, coincubation with the blocking peptide prevented Ca_v3.1 immunofluorescence. Bottom right, positive PECAM-1 immunofluorescence is shown in blue. Ca_v3.1 and Sytox nuclear stain is in white in all cases.