Fig. 1.

Inhibition profile of mOat6. Inhibition of mOat6-mediated uptake of [³H]ES (5 μM) by DHEAS, HA, indoxyl sulfate, 5-HIAA, HVA, DOPAC, gatifloxacin, ofloxacin, norfloxacin, ciprofloxacin, 5-CH3-THF, leucovorin, folate, methotrexate, and probenecid (500 μM) was measured in CHO-mOat6 cells (15 min). Background ES accumulation was measured in CHO-FRT cells in the absence of inhibitor and is shown to provide a clear gauge of the low background noise in the experimental system. Raw accumulation values for CHO-FRT and CHO-mOat6 cells were 0.061 ± 0.013 versus 0.428 ± 0.010 pmol/μg protein/15 min, respectively. Values are mean ± S.E. of triplicate values. *, p < 0.05; **, p < 0.01; ***, p < 0.001.