Table 1. Protein components of RNA polymerase II and III complexes.
| Complex | Saccharomyces cerevisiae | Sequence conservation | Leishmania major geneb | Predicted sizea | Peptides identifiedc | |||
|---|---|---|---|---|---|---|---|---|
| subunit | sizea | name | systematic ID | LmRPB2 | LmRPB6 | |||
| RNAP II | B220 | 192 | +++ | RPB1 | LmjF31.2610 | 184.7 | 29(6) | 17(6) |
| B150 | 139 | +++ | RPB2 | LmjF31.0160 | 133.8 | 21(6) | 15(5) | |
| B44 | 35 | ++ | RPB3 | LmjF29.0140 | 37.5 | 7(6) | 5(3) | |
| B32 | 25 | + | RPB4 | (LmjF29.0400) | 18.0 | |||
| B16 | 19 | ++ | RPB7 | LmjF32.1280 | 21.9 | 2(2) | 1(1) | |
| B12.6 | 14 | ++ | RPB9 | LmjF28.0120 | 15.6 | |||
| B12.5 | 14 | ++ | RPB11 | LmjF27.1550 | 14.8 | 4(3) | ||
| Sharedd | ABC27 | 25 | ++ | RPB5 | LmjF18.0780 | 27.2 | 4(4) | 4(3) |
| RPB5z | LmjF18.0790 | 37.6 | ||||||
| ABC23 | 18 | ++ | RPB6 | LmjF34.0890 | 17.5 | 1(1) | ||
| RPB6z | LmjF25.0140 | 14.2 | ||||||
| ABC14.5 | 17 | + | RPB8 | LmjF28.0810 | 16.5 | 1(1)e | ||
| ABC10α | 8 | ++ | RPB12 | (LmjF20.0490) | 17.1 | |||
| ABC10β | 8 | ++ | RPB10 | LmjF13.1120 | 9.6 | 1(1) | ||
| RPB10z | LmjF25.1315f | 9.8 | ||||||
| RNAP III | C160 | 162 | +++ | RPC1 | LmjF34.0360 | 173.6 | 8(1) | |
| C128 | 129 | +++ | RPC2 | LmjF20.0010 | 126.8 | 4(1) | ||
| C82 | 74 | + | RPC3 | (LmjF27.2600) | 65.4 | 3(1) | ||
| (LmjF02.0680g) | 41.4 | |||||||
| C53 | 46 | + | RPC4 | (LmjF35.4170) | 37.9 | 1(1)h | ||
| C37 | 32 | + | RPC5 | (LmjF13.1370) | 76.5 | 4(1) | ||
| C34 | 36 | ++ | RPC6 | LmjF29.1320 | 48.1 | 1(1) | ||
| C31 | 28 | + | RPC7 | NFi | ||||
| C25 | 24 | ++ | RPC8 | LmjF09.1060 | 27.0 | |||
| C17 | 18 | + | RPC9 | (LmjF03.0790) | 45.4 | 2(1) | ||
| C11 | 12 | ++ | RPC10 | LmjF19.0420 | 16.3 | |||
| Sharedj | AC40 | 37 | +++ | RPAC1 | LmjF19.0660 | 47.3 | 2(1) | |
| AC19 | 16 | ++ | RPAC2 | LmjF28.2060 | 15.2 | 1(1) | ||
a
Molecular weight in kDa
b
Genes in parenthesis show weak similarity by Blast
c
The first number indicates the number of different peptides detected with each of the tandem affinity purification-tagged constructs, the number in parenthesis shows the number of experiments in which peptides were detected
d
Subunits shared between RNAPI, II and III in yeast and human
e
This peptide was identified by manual analysis of the Sequest output files, not by PeptideProphet.
f
Not in original L. major genome annotation; found by tBlastN analysis using Tb927.3.1250
g
LmjF02.0680 is probably a truncated pseudogene
h
Only a non-tryptic peptide was detected
i
Leishmania major orthologue not yet identified
j
Subunits shared between RNAPI and III in yeast and human