Table 2. Proteins co-purifying with RNA polymerase II and III complexesa.
| Leishmania major gene | Predicted sizeb | Peptides detectedc | Possible function | |
|---|---|---|---|---|
| LmRPB2 | LMRPB6 | |||
| LmjF32.0750 | 25.1 | 6(2) | 3(3) | RNA binding protein |
| LmjF17.0010 | 87.6 | 3(2) | 3(4) | Hypothetical protein; Tryp-specific |
| LmjF07.0870 | 41.8 | 3(2) | 2(2) | Splicing factor PTSR-1 |
| LmjF29.0020 | 114.8 | 3(1) | 3(2) | Transcription elongation factor (FACT 140) |
| LmjF10.0870d | 14.6 | 4(1) | 3(1) | Histone H3 |
| LmjF34.0930 | 12.5 | 2(2) | 3(1) | Hypothetical protein; conserved |
| LmjF06.0010 | 11.3 | 1(1) | 4(2) | Histone H4 |
| LmjF14.1440 | 103.9 | 1(1) | 3(2) | Hypothetical protein; Tryp-specific |
| LmjF25.0590 | 19.7 | 1(1) | 3(2) | Hypothetical protein; Tryp-specific |
| LmjF08.0430 | 100.1 | 1(1) | 2(1) | Hypothetical protein; Tryp-specific |
| LmjF30.2620 | 77.9 | 1(2) | 1(1) | Hypothetical protein; Tryp-specific |
| LmjF08.1222e | 11.4 | 1(1) | 1(1) | Hypothetical protein; Tryp-specific |
| LmjF32.0400 | 66.8 | 1(1) | 1(1) | DEAD/H box RNA helicase |
| LmjF29.2090 | 175.9 | 7(1) | Helicase | |
| LmjF35.1310f | 11.4 | 4(2) | Histone H4 | |
| LmjF09.1340g | 12.2 | 4(1) | Histone H2B | |
| LmjF10.0160 | 54.6 | 3(1) | Chromosome segregation ATPase? | |
| LmjF35.0370 | 46.4 | 2(1) | DEAD box RNA Helicase | |
| LmjF27.0840 | 95.3 | 2(2) | Hypothetical protein; conserved | |
| LmjF25.1550 | 11.6 | 2(2) | Hypothetical protein; Tryp-specific | |
| LmjF36.3050 | 15.8 | 2(2) | Hypothetical protein; Tryp-specific | |
| LmjF34.4100 | 35.2 | 2(2) | Hypothetical protein; Tryp-specific | |
| LmjF07.1110 | 40.5 | 2(2) | Hypothetical protein; Tryp-specific | |
| LmjF09.0050 | 29.4 | 2(1) | Endonuclease III | |
| LmjF07.0220 | 67.1 | 2(1) | Hypothetical protein; Tryp-specific | |
| LmjF26.1560 | 177.1 | 1(2) | Helicase | |
| LmjF32.0150 | 111.0 | 1(2) | Small nucleolar ribonucleoprotein? | |
Only proteins with more than one unique peptide detected or the same peptide detected in more than one experiment are shown. Proteins likely to be contaminants (including several ribosomal proteins, translation factors, heat shock proteins, α- and β-tubulin, paraflagellar rod protein and a number of mitochondrial proteins) were not included.
in kDa
The first number indicates the number of unique peptides detected with each of the tandem affinity purification-tagged constructs, the number in parenthesis shows the total number of experiments the peptide was observed in.
Also matches LmjF10.0990, LmjF16.0575, LmjF16.0570 and LmjF16.0610
Also matches LmjF08.1260
Also matches LmjF31.3180, LmjF36.0020, LmjF02.0020 and LmjF25.2450
Also matches LmjF19.0050, LmjF19.0030 and LmjF19.0040