Skip to main content
. 2010 Aug 3;25(10):2405–2414. doi: 10.1093/humrep/deq183

Figure 4.

Figure 4

(A) Immunoexpression (brown) of OCT4 (top), VASA (middle) and MAGE-A4 (bottom) in germ cells in a first trimester human testis prior to grafting (Pre-graft control) and 6 weeks after grafting into a mouse host (graft). Note that after grafting, the proportion of germ cells expressing OCT4 decreases as demonstrated by the presence of unstained germ cells (arrowhead), compared with the pre-graft control, whereas expression of MAGE-A4 and VASA (arrows, inset) increase (neither are expressed in the pre-graft control). Scale bar = 50 µm. (B) Quantification of germ cell differentiation in human fetal testis xenografts. The proportion of germ cells expressing OCT4 (red bars) or VASA (blue bars) in first (upper panels) and second (lower panels) trimester human fetal testes for pre-graft control, grafts and equivalent non-grafted controls. Values are Mean ± SEM for n = 3 fetuses. *P < 0.05, **P < 0.01, ns, not significant.