Table II.

Antibodies and conditions for triple immunofluorescence in xenograft study using human fetal testis tissue in nude mice.

Antigen	Dilution	Secondary antibody	Visualization
OCT4	1:150	aCAG-p	cTyr-Cy3 (10 min)
MAGE-A4	1:100	bCAM-p	dTyr-Cy5 (10 min)
Ki67	1:200	bCAM-p	eTyr Fluor (10 min)

^aCAG-p, Chicken anti-goat peroxidase (Sigma, Poole, UK).

^bCAM-p, Chicken anti-mouse peroxidase (Sigma, Poole, UK).

^cTyr Cy3, Tyramide Cy3 (Perkin Elmer, MA, USA).

^dTyr Cy5, Tyramide Cy5 (Perkin Elmer).

^eTyr Fluor, Tyramide Fluorescien (Perkin Elmer).