Figure 3.

(A, B) AREG production and secretion is specifically mediated by LH signaling. Primary cell cultures were established from luteinized human granulosa cells obtained at the time of OR. Cells were then stimulated by either recombinant human FSH, hCG or vehicle only for up to 60 h. Cultured medium was assayed for AREG (Fig. 3A) or progesterone (Fig. 3B). Results for AREG production are mean ± SEM values from three different primary cell cultures taken from different patients. Statistical significance is in comparison with the no gonadotrophin stimulation at 24 h. Progesterone results for the primary granulosa cell culture are from an individual patient.