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. 2010 Aug 18;25(10):2569–2578. doi: 10.1093/humrep/deq212

Figure 4.

Figure 4

(A) Biologically active AREG in human FF causes CC expansion. Mouse COCs were incubated with 10% human FF (post-hCG) which was immunodepleted (AB) of various EGF-like growth factors. AREG depletion specifically blocks normal CC expansion in this assay. Recombinant AREG (rAREG) is included as a control. Magnification: ×10. (B) Biologically active AREG in human FF causes CC expansion. A histogram showing the mean ± SEM of nine or more COCs analyzed in three separate experiments. Statistical significance determined relative to the mock immunoprecipitated 10% FF. (C) Human FF sampled before hCG administration (pre-hCG FF) does not promote COC expansion. (D) AREG in human FF induces oocyte nuclear maturation. Mouse COCs were incubated with 10% human FF (post-hCG) and assessed for nuclear maturation by light microscopy. Results are mean ± SEM of 15 or more COCs analyzed in three separate experiments. AREG IP, AREG removed by immunoprecipitation.