Figure 1.

Western blotting using anti-intelectin antibodies. (A) Recombinant human intelectin-1 (5 ng per lane) was resolved by SDS–PAGE under the non-reducing (NR) or the reducing (R) conditions and detected by western blotting using mAb or affinity-purified anti-intelectin pAb. (B) Recombinant intelectin-1 (rITLN1) and recombinant intelectin-2 (rITLN2) were purified with galactose-Sepharose from culture supernatant of transiently intelectin-transfected RK-13 cells. Serum intelectin-1 (Serum ITLN1) was purified from the serum of healthy human donors. The samples were detected by western blotting using anti-intelectin pAb, as described in the Materials and Methods.