Figure 2.

Production of intelectin-1 in MPM cell lines. (A) Intelectin-1 was purified with galactose-Sepharose from 3 days of culture supernatant containing 10% fetal bovine serum and was detected as a single 120 kDa band by non-reducing western blotting. Intelectin-1-transfected RK-13 cells (ITLN-RK13) were cultured in medium containing 5% fetal bovine serum. Results were obtained by reprobing and reblotting of an identical membrane as described in the Materials and Methods. Serum intelectin-1 (Serum ITLN1) was prepared from the serum of healthy human donor. (B) Intelectin-1 mRNAs of two MPM cell lines were compared using semiquantitative RT–PCR. A sample without RNA was used as a negative control (NC). (C) Intelectin-1 was purified with galactose-Sepharose from 5 days of culture supernatant containing 10% fetal bovine serum and was detected by non-reducing western blotting using affinity-purified anti-intelectin pAb.