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. 2010 Jul 13;103(4):517–523. doi: 10.1038/sj.bjc.6605786

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Copyright © 2010 Cancer Research UK

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Western blotting of intelectin-1 in pleural effusion and plasma. Recombinant intelectin-1 (rITLN1) is used as a positive control. Mesothelioma patients of Y1, Y2, Y4, Y5, Y6, Y7, and G1 are identical to the ones in Table 1, respectively. (A) Plasma (0.5 μl) was analysed by non-reducing western blotting using affinity-purified anti-intelectin pAb. (B) Pleural effusion (0.5 μl) was analysed by non-reducing western blotting using affinity-purified anti-intelectin pAb or 15:3G9. Results were obtained by reprobing and reblotting of an identical membrane as described in the Materials and Methods.