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. 2010 Sep 7;123(19):3401–3411. doi: 10.1242/jcs.068684

Fig. 8.

Fig. 8.

Ex vivo FACS analysis using the mouse model of trypanosomiasis. Mice were inoculated with transgenic (BSF K9.K69) parasites induced (+DOX) or not induced (−DOX) for the ectopic expression of ESAG9-K9 and ESAG9-K69. Controls were wild-type monomorphic parasites (WT) or naïve inoculations (Media). The following traits were assayed: (A) the percentage of live granulocytes that were Gr-1-positive (neutrophils); (B) the percentage of live granulocytes that were Siglec F-positive (eosinophils); (C) the percentage of live lymphocytes that were CD4+; (D) the percentage of CD4+ T-cells that were undergoing apoptosis (assayed by Annexin V); (E) the percentage of CD4+ T-cells that were expressing IFN-γ, as assayed by intracellular cytokine staining; and (F) the percentage of CD8+ T-cells that were expressing IFN-γ.