Skip to main content
. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Microbiol. 2010 Jun 1;77(3):755–770. doi: 10.1111/j.1365-2958.2010.07244.x

Fig. 1. Comparison of autoagglutination, pilin expression, pilus assembly and TCP:TCP interaction patterns for classical and El Tor V. cholerae biotypes.

Fig. 1

(A) Autoagglutination of overnight V. cholerae cultures grown under TCP-inducing conditions: wild type classical strain O395 and O395 expressing El Tor TcpA (RT4340) autoagglutinate well (+++) whereas El Tor strain C6706 and the classical mutant strain ML10 (C120A) do not autoagglutinate (-). (B) Immunoblots of TcpA in whole cell cultures (upper panel) and sheared cell supernatant (lower panel) detected with polyclonal anti-TcpA antibody (TcpA6, residues 174-199, (Sun et al., 1991)) to assess TcpA expression and TCP assembly, respectively. (C) Morphology and bundling characteristics of V. cholerae TCP as assessed by negative stain TEM of whole cell cultures (upper panels) and partially purified pili (lower panels). Scale bars, 100 nm.