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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Microbiol. 2010 Jun 1;77(3):755–770. doi: 10.1111/j.1365-2958.2010.07244.x

Fig. 4. Analysis of classical-to-El Tor mutants for pilin and pilus expression and pilus morphologies and interaction patterns.

Fig. 4

(A) Immunoblots of total TcpA in whole cell cultures (upper panel) and TCP in sheared cell supernatant (lower panel) for tcpA mutants and control strains, detected with polyclonal anti-TcpA antibody (TcpA6). All mutants, with the exception of strain ML10, produced TcpA and TCP at or above wild type levels, indicating that the mutations do not aberrantly affect pilin fold or pilus assembly. (B) TEM images of TCP from whole cell cultures of V. cholerae strains harboring the single A156D change, ML15, the complete classical-to-El Tor D-region mutant LC16, the D156A reversion of LC16, strain ML25, and the D113A mutant, ML9. TCP from strains ML15 and ML9 display a mixed pattern of crosshatches and rope-like bundles, whereas LC16 pili are predominantly crosshatched and ML25 TCP form mostly rope-like bundles. Scale bars, 100 nm.