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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Microbiol. 2010 Jun 1;77(3):755–770. doi: 10.1111/j.1365-2958.2010.07244.x

Table 2.

Phenotypes of V. cholerae tcpA mutants

Strain1 TcpA alteration Residues in D-region2 Pilin Pili AI3 AS4 Pilus:pilus interactions
O395 Wild type, classical AAEKDK + + 1.00 +++ bundles
C6706 Wild type, El Tor EDAANT + + 0.02 - crosshatch/bundles
RT4340 O395, tcpAET EDAANT + + 0.53 +++ crosshatch/bundles
ML7 RT4524, tcpACl AAEKDK + + 0.84 +++ bundles
ML10 C120A AAEKDK -/+ - 0.05 - -
D-region alterations
LC1 A138E------------------------------------------------- EAEKDK + + 0.53 +++ bundles
ML15 ----------A156D--------------------------------------- ADEKDK + + 1.27 +++ bundles/crosshatch
ML17 --------------------E158A----------------------------- AAAKDK + + 2.09 ++++ bundles
ML18 ------------------------------K172A------------------- AAEADK + + 2.09 ++++ bundles
ML12 ----------------------------------------D175N--------- AAEKNK + + 0.17 + bundles
LC6 --------------------------------------------------K187T AAEKDT + + 1.9 ++++ bundles
ML14 ------------------------------K172A/D175N--------- AAEANK + + 1.16 +++ bundles
LC7 ----------A156D/E158A----------------------------- ADAKDK + + 0.97 +++ bundles/crosshatch
LC9 A138E/A156D/E158A------------------------------ EDAKDK + + 0.58 +++ bundles/crosshatch
LC10 A138E/A156D/E158A/K172A-------------------- EDAADK + + 0.11 + bundles/crosshatch
LC11 A138E/A156D/E158A---------------------K187T EDAKDT + + 0.11 + bundles/crosshatch
LC12 ----------A156D/E158A/K172A/D175N--------- ADAANK + + 0.72 +++ bundles/crosshatch
LC13 A138E/A156D/E158A/K172A/D175N---------- EDAANK + + 0.04 - bundles/crosshatch
LC15 ----------A156D/E158A/K172A/D175N/K187T ADAANT + + 1.52 ++++ bundles/crosshatch
ML16 A138E/A156D/E158A/K172A-----------K187T EDAADT + + 0.04 - bundles/crosshatch
LC16 A138E/A156D/E158A/K172A/D175N/K187T EDAANT + + 0.04 - crosshatch
ML25 A138E-----------E158A/K172A/D175N/K187T EAAANT + + 0.47 ++ bundles
Cavity alterations
ML28 D113G + + 0.04 - bundles
ML9 D113A + + 0.07 - bundles/crosshatch
Combined D-region/cavity mutants
DN1 D113G/A138E/A156D/E158A/K172A/K187T EDAADT + + 0.08 - crosshatch
DN2 D113A/A138E/A156D/E158A/K172A/K187T EDAADT + + 0.08 - crosshatch
1

All strains contain pMT5.

2

D-region residues 138, 156, 158, 172, 175 and 187 are listed; underlined residues are the classical-to-El Tor changes; non-underlined residues are the unchanged classical residues; residue 156 is shown in bold type.

3

AI, autoagglutination index, reported as at fraction of wild type O395 autoagglutination, calculated as the ratio of the inverse of the test supernatant OD600 over the inverse of O395 supernatant OD600 for n=3.

4

AS, autoagglutination scale: (-), AI of 0-0.10; (+), 0.11-0.20, detectable autoagglutination; (++), 0.21-0.50, moderate autoagglutination; (+++), 0.51-1.50, good or “wild type” (O395) autoagglutination; (++++), ≥1.51, better than wild type autoagglutination (clear supernatant).