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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Biotechniques. 2007 Jul;43(1):64–71. doi: 10.2144/000112492

Figure 2. Evidence for specificity of primary and secondary antibodies.

Figure 2

(A–E) Red (Alexa Fluor 633) or green (Alexa Fluor 488) fluorescence. Bromodeoxyuridine (BrdU) was detected by both the (A) anti-BrdU antibody and the (B) anti-BrdU/IdU antibody; iododeoxyurdine (IdU) was essentially undetected with the (C) anti-BrdU antibody, while being detected by the (D) anti-BrdU/IdU antibody. Alexa Fluor 633 goat anti-rat immunoglobulin G (IgG) had a limited cross-reactivity with (E) mouse anti-BrdU/IdU antibody, while Alexa Fluor 488 goat anti-mouse showed no reactivity with the (F) rat anti-BrdU antibody. Data for one of two replicate experiments is shown.