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. 2010 Sep 15;5(9):e12746. doi: 10.1371/journal.pone.0012746

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Jögi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunoblot assay with plasminogen (lane 1), plasmin (lane 2), murine plasma (lane 3), and wound extracts 7 days post wounding obtained from wild type mice (wt, lane 4), control mAb-treated (i.e. anti-TNP) tPA-deficient mice (lanes 5–7), mU1-treated tPA-deficient mice (lanes 8–10), uPA;tPA double-deficient mice (lane 11), and Plg-deficient mice (lane 12). Plasminogen and plasmin were detected using a polyclonal rabbit anti-human plasminogen antibody. #1, #2, #3 denote wound extracts from three different mice.