Figure 6. Transcript expression analysis by qRT-PCR in response to HS and BR treatment.

(A) For HS treatment, 10 day-old Arabidopsis seedlings were exposed to 38°C for 1 and 3 h at which time the plant tissue above the medium was collected and quick-frozen for RNA isolation. (B) BR treatment was given to 21 day-old seedlings for 3 and 12 h and the tissue was collected as in A. Transcripts were analyzed by qRT-PCR. Bars indicate mean ± SD.