Figure 5. Regulatory T cell response to MOG recall in early disease.

Day 7 p.i. lymph node cells from DA and DA.PVG-Eae23 were stained for T reg markers and pre-gated for CD4⁺ T cells using flow cytometry. A) Representative flow cytometry plot of CD4 (x-axis) and CD25 (y-axis) showing the gate for the CD4⁺CD25⁺ population. B) Representative flow cytometry plot of Foxp3 (x-axis) and CD25 (y-axis) showing the gate for the CD4⁺CD25⁺Foxp3⁺ population. C) Fold change of CD4⁺CD25⁺Foxp3⁺ sub-populations after MOG recall compared to ex vivo. Median values are indicated by horizontal bars. Mann-Whitney U-test was used to compare the fold change; p values ≤0.05 were considered significant, **  = p≤0.01. Abbreviations: D.P-Eae23 =  DA.PVG-Eae23.