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. 2010 Sep 15;5(9):e12766. doi: 10.1371/journal.pone.0012766

Figure 1. ADAM8 is expressed in thymic stromal cells and thymocytes.

Figure 1

Relative ADAM8 RNA levels as determined by qrtPCR in (A) total thymic tissue, thymic hematopoietic cells (CD45+, EpCAM−), epithelial cells (CD45−, EpCAM+), endothelial cells (CD45−, EpCAM−, CD31+) and other non-epithelial stromal cells (EpCAM−, CD45−, CD31−), (B) thymic T cell subsets and (C) TEC. FACS sorted cells (>95% purity) were analyzed for ADAM8 RNA expression using the reference gene HPRT. ADAM8 RNA values are expressed relative to HPRT expression. Data are presented as mean values and error bars as SEM. Data are from at least two independent experiments (n≥3). (D–G) Reporter gene (β-galactosidase) expression in ADAM8−/− thymic sections detected by immunofluorescence. Thymic sections were stained with antibodies directed against β-galactosidase to reveal potential ADAM8 expression and (D) β5t marking cTEC, (E) MTS10 marking mTEC, (F) ER-TR7 marking fibroblasts and (G) KT-3 marking T cells. Scales bar 50 µm. Data are from at least four independent experiments (n≥4).