Figure 2. Mutations in wnt2 Affect Other Presynaptic Proteins.

A–B: Confocal images of third instar 6/7 NMJs immunolabeled with α-synaptotagmin (Syt, A) and α-synaptobrevin (Syb, B)with quantification of fluorescence (right histograms). Scale bar  = 20 µm. C: Relative Brp mRNA levels in 1^st instar (left), 2^nd instar (middle), and 3^rd instar (right) larvae, measured by quantitative RT-PCR.