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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Pigment Cell Melanoma Res. 2010 Apr 23;23(5):627–634. doi: 10.1111/j.1755-148X.2010.00718.x

Figure 3.

Figure 3

WT and mutant melanocytes were transfected with an expression plasmid encoding an HA-tagged Atf6 protein for the purpose of monitoring Golgi-based protein cleavage. (A) Transfected WT melanocytes were subjected to either 15nM thapsigargin or 10μg/mL tunicamycin, while (B) transfected mutant cells were dosed with thapsigargin for 24 hours. Western blot analysis was used to determine Atf6 cleavage and expression of its downstream target BiP. (C) WT cells were treated with 15mM thapsigargin over an eight day period and BiP expression was monitored by Western blot analysis.