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WT melanocytes were treated with 150nM thapsigargin. Cells were lysed and proteins harvested for analysis at the indicated time points. Samples were normalized for protein concentration prior to analysis and subjected to Western blot analysis to detect expression and phosphorylation of Bcl2 and Caspase 12 cleavage. Actin is shown as a loading control. Mebendazole, previously shown to trigger phosphorylation of Bcl2 at Serine 70 was used as a positive control (Doudican et al., 2008).
