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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Cell Physiol. 2010 Nov;225(3):682–691. doi: 10.1002/jcp.22264

FIGURE 2. TNFα/NF-κB signalling promotes MS formation and invasive capacity of breast cancer cells via SLUG up-regulation.

FIGURE 2

(A): MS forming capacity of empty/pSLUG transiently transfected (1µg, 24h) MCF7 cells and pCtoGMB/ shSLUG stably transduced MCF7 cells exposed or less to TNFα (10 ng/ml, 24h); (B): MS forming capacity of MCF7 cells exposed to the IκBα degradation inhibitors Parthenolide or Bay 11-7082 (5µM, 24h each), the specific IKKβ inhibitor sc-514 (5µM, 24h each), or stably transduced with an IKKβ specific shRNA/empty expressing retroviral vector; (C): MS forming capacity of pCtoGMB/shSLUG MCF7 cells transduced with empty or p65/IKKβ-CA encoding vector, representative MS pictures are also reported. The scale bar inset corresponds to 100µm; (D): Invasion assay in pSLUG transfected (1µg), TNFα exposed (10ng/ml, 24h), p65/IKKβ-CA transduced pCtoGMB/shSLUG MCF7 cells. Data are presented as mean +/− S.D. of three replicates, p values of unpaired t tests: *<0.05, #<0.01 and § <0.005. The scale bar represents 100 µm.